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Author Sumi, M. G. ♦ Mathai, A. ♦ Reuben, S. ♦ Radhakrishnan, V. V. ♦ Sasikumar, S. ♦ Jayapal, V. ♦ Felix, J.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Indian Journal of Experimental Biology
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health ♦ Diseases
Subject Domain (in MeSH) Bacterial Infections and Mycoses ♦ Diseases ♦ Diagnosis ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment
Subject Keyword Laboratory Diagnosis
Abstract IgG antibody to Mycobacterium tuberculosis from the sera of patients with 'definite' pulmonary tuberculosis (PT) was isolated and coupled with Cyanogen bromide-Sepharose 4B. Using an immunoabsorbent affinity chromatography, 14 kDa antigen was recovered from the culture filtrates of M. tuberculosis. With this mycobacterial antigen, a dot immunobinding assay (Dot-Iba) was developed for the detection of specific antibody to M. tuberculosis in the sera of patients with PT and controls. The assay gave positive results in all the 12 sputum-smear positive [acid fast bacilli (AFB)] patients with PT and gave negative results in the 50 sera from control groups. The Dot-Iba as described in this study, is simple, rapid and specific for laboratory diagnosis of PT.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal Indian journal of experimental biology
Volume Number 39
Issue Number 10
Page Count 977
Starting Page 8
Ending Page 984