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Author Sandhyamani, S. ♦ Sindhu, J. K. ♦ Sriramachari, S.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Virchows Archiv
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Domain (in MeSH) Chemical Phenomena ♦ Biological Sciences
Abstract Romhanyi's technique for preservation and restoration of color in museum specimens is almost unknown outside Hungary. The technique was based on the use of pyridine, nicotine and sodium dithionite (sodium hydrosulphite) for restoring the natural red color of formalin-fixed gross specimens. We developed a modification of the original technique using a mounting medium containing citrate-phosphate-dextrose (CPD) buffer and glycerin to control pH and osmolality. Specimens recolorized with the modified and improved Romhanyi technique remained stable with a natural red color, and no histological deterioration, for more than 18 years. The technique demonstrates the formation of red colored complexes between nicotine and pyridine and ferro-hemochromes such as hemoglobin, myoglobin and other cytochromes in tissues, giving specimens a natural color. The modified technique enables further improvement for the old process of recolorization of museum specimens. In addition, it opens up new methods for demonstration of nicotine, pyridine and their analogues in biological tissues.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal VIRCHOWS ARCHIV
Volume Number 447
Issue Number 1
Page Count 5
Starting Page 94
Ending Page 98