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Author Sumi, M. G. ♦ Mathai, A. ♦ Reuben, S. ♦ Sarada, C. ♦ Radhakrishnan, V. V.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Clinical and Diagnostic Laboratory Immunology
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health ♦ Diseases
Subject Domain (in MeSH) Bacterial Infections and Mycoses ♦ Diseases ♦ Diagnosis ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Immune System Phenomena ♦ Biological Sciences
Subject Keyword Immunology
Abstract A simple immunocytochemical method was standardized for the direct demonstration of mycobacterial antigen in cerebrospinal fluid (CSF) specimens of patients with tuberculous meningitis (TBM). CSF-cytospin smears were prepared from 22 patients with a clinical diagnosis of TBM and also from an equal number of patients with nontuberculous neurological diseases (disease control). Immunocytological demonstration of mycobacterial antigens in the cytoplasm of monocytoid cells was attempted, by using rabbit immunoglobulin G to Mycobacterium tuberculosis as the primary antibody. Of the 22 CSF-cytospin smears from TBM patients, 16 showed positive immunostaining, while all of the CSF-cytospin smears from the disease control showed negative immunostaining for mycobacterial antigen. The technical aspects of this immunocytological method for the demonstration of mycobacterial antigens are simple, rapid, and reproducible, as well as specific, and therefore can be applied for the early diagnosis of TBM, particularly in patients in whom bacteriological methods did not demonstrate the presence of M. tuberculosis in the CSF.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Volume Number 9
Issue Number 2
Page Count 4
Starting Page 344
Ending Page 347