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Author Anie, Y. ♦ Sumi, S. ♦ Varghese, Philip ♦ Madhavi, Latha G. K. ♦ Sathish, M. ♦ Radhakrishnan, V. V.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Diagnostic Microbiology and Infectious Disease
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health ♦ Diseases
Subject Domain (in MeSH) Immune System Phenomena ♦ Biological Sciences
Subject Keyword Immunology
Abstract The conventional bacteriologic methods used for diagnosing pleural tuberculosis are less sensitive and time consuming. The objective of this study was to develop nonbacteriologic methods and to assess their potential utilities for the rapid diagnosis, especially in smear/culture-negative patients. One hundred forty patients with pleural effusion were investigated for tuberculous etiology by bacteriologic methods. Mycobacterium tuberculosis in the pleural fluid specimens was isolated in I I patients. To establish a tuberculous etiology in the remaining 129 patients, we performed the following assays: a) estimation of tuberculosis-associated glycolipid antigen (TBGL) by a modified indirect enzyme-linked inummosorbent assay (ELISA), b) an immunocytochemical method for the demonstration of TBGL antigen in the Cytospin smears, and c) detection of mycobacterial DNA by polymerase chain reaction (PCR). Estimation of TBGL antigen by ELISA showed 100% specificity and overall 85.5% sensitivity. Immunocytochemistry could be applied only in those samples with adequate number of macrophages. PCR carried sensitivity and specificity of 87% and 93%, respectively. Estimation of TBGL antigen in pleural fluid specimens by ELISA has a definite role in establishing tuberculous etiology, particularly in those patients in whom bacteriologic methods did not demonstrate M. tuberculosis and also in those in whom a distinction between tuberculous and nontuberculous etiology was not possible based on the clinical and radiologic features of the thorax. (c) 2007 Elsevier Inc. All rights reserved.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Volume Number 59
Issue Number 4
Page Count 6
Starting Page 389
Ending Page 394