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Author Divya, P. ♦ Krishnan, L. K.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Journal of Tissue Engineering and Regenerative Medicine
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health ♦ Human physiology
Subject Domain (in MeSH) Tissues ♦ Cells ♦ Anatomy ♦ Amino Acids, Peptides, and Proteins ♦ Biomedical and Dental Materials ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Equipment and Supplies ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment
Subject Keyword Tissue Engineering
Abstract The success of a biocompatible vascular graft depends upon its mechanical attributes and postimplantation healing responses. Mechanical strength is a paramount issue because grafts placed in the arterial circulation must be capable of withstanding long-term haemodynamic stress without graft failure. Extracellular matrix (ECM) proteins that are deposited by the cells to remodel the environment play a major role in determining the construct stability and strength. A suitable scaffold that stimulates ECM deposition and remodelling by cells grown in vitro may generate tissues with normal function. The objective of this study was to prove that fibrin matrix composition can be modified with growth factors (GFs) and glycosaminoglycans (GAGs) to promote ECM remodelling coupled with endothelial cell (EC) growth. Effect of GFs and GAGs on ECM production and remodelling was studied separately and in combination. Matrices recovered after EC cultures were analysed after immunochemical staining and it was observed that GFs and GAGs influence collagen IV and elastin deposition. Quantitative PCR analysis of mRNA after specific periods of culture demonstrated significant upregulation of elastin and collagen expression in EC by combination of GFs and GAGS when compared to their individual effects. The results of experiments conducted with various combinations of GFs and GAGs show that a biomimetic approach of immobilization of signalling molecules in fibrin can upregulate ECM remodelling with simultaneous degradation of the fibrin matrix and deposition of collagen IV and elastin. Hence, this combination may be suitable for cardiovascular tissue generation in vitro. Copyright (C) 2009 John Wiley & Sons, Ltd.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Volume Number 3
Issue Number 5
Page Count 12
Starting Page 377
Ending Page 388