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Author Madathil, Bernadette K. ♦ Lin, Qingsong ♦ Hew, Choy-leong ♦ Mohanty, M.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Journal of Orthopaedic Research
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Domain (in MeSH) Immune System Phenomena ♦ Biological Sciences
Subject Keyword Biocompatibility
Abstract Periprosthetic osteolysis leading to asceptic loosening remains the primary cause of failure of Joint replacement Although many inflammatory cell types have been implicated, the exact pathomechanisms of asceptic loosening have not been delineated In the present study we have adopted a proteomic approach to elucidate the initial signals that are expressed to particulate material, using an in vitro cell culture system Human lung fibroblasts MRC-5 were cultured with Cobalt Chromium (CoCr ASTM F-75, 1-7 pm) particles Cells were harvested after 72 h incubation and total cellular proteins extracted for downstream analysis via 2D Gel Electrophoresis and tandem mass spectrometry using MALDI-TOF-TOF-MS Thirteen protein spots showed greater than twofold increase, following 72 h incubation of fibroblast with CoCr particles Four of these proteins were identified by tandem mass spectrometry These were Annexin II, Pyruvate kinase, Triose phosphate isomerase, and N-myc downstream regulated gene 1 protein Cobalt is a hypoxia mimicking agent and N-myc downstream regulated gene 1 protein, Triose phosphate isomerase, Pyruvate kinase, and Annexin II are important hypoxia regulated gene products that are found to be over expressed in cellular oxidative stress response Our data indicates that exposure of fibroblast to CoCr alloy induces the transition of these cells into a hypoxia like state and oxidative stress even in normoxic culture conditions The study reflects the possibility of the presence of a hypoxic environment in the periprosthetic tissue surrounding metallic implants (C) 2010 Orthopaedic Research Society Published by Wiley Periodicals, Inc J Orthop Res 28 1360-1367, 2010
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal JOURNAL OF ORTHOPAEDIC RESEARCH
Volume Number 28
Issue Number 10
Page Count 8
Starting Page 1360
Ending Page 1367