|Author||Madathil, Bernadette K. ♦ Lin, Qingsong ♦ Hew, Choy-leong ♦ Mohanty, M.|
|Source||Sree Chitra Tirunal Institute for Medical Sciences & Technology|
|Publisher||Journal of Orthopaedic Research|
|Subject Domain (in DDC)||Technology ♦ Medicine & health|
|Subject Domain (in MeSH)||Immune System Phenomena ♦ Biological Sciences|
|Abstract||Periprosthetic osteolysis leading to asceptic loosening remains the primary cause of failure of Joint replacement Although many inflammatory cell types have been implicated, the exact pathomechanisms of asceptic loosening have not been delineated In the present study we have adopted a proteomic approach to elucidate the initial signals that are expressed to particulate material, using an in vitro cell culture system Human lung fibroblasts MRC-5 were cultured with Cobalt Chromium (CoCr ASTM F-75, 1-7 pm) particles Cells were harvested after 72 h incubation and total cellular proteins extracted for downstream analysis via 2D Gel Electrophoresis and tandem mass spectrometry using MALDI-TOF-TOF-MS Thirteen protein spots showed greater than twofold increase, following 72 h incubation of fibroblast with CoCr particles Four of these proteins were identified by tandem mass spectrometry These were Annexin II, Pyruvate kinase, Triose phosphate isomerase, and N-myc downstream regulated gene 1 protein Cobalt is a hypoxia mimicking agent and N-myc downstream regulated gene 1 protein, Triose phosphate isomerase, Pyruvate kinase, and Annexin II are important hypoxia regulated gene products that are found to be over expressed in cellular oxidative stress response Our data indicates that exposure of fibroblast to CoCr alloy induces the transition of these cells into a hypoxia like state and oxidative stress even in normoxic culture conditions The study reflects the possibility of the presence of a hypoxic environment in the periprosthetic tissue surrounding metallic implants (C) 2010 Orthopaedic Research Society Published by Wiley Periodicals, Inc J Orthop Res 28 1360-1367, 2010|
|Education Level||UG and PG|
|Learning Resource Type||Article|
|Educational Framework||Medical Council of India (MCI)|
|Journal||JOURNAL OF ORTHOPAEDIC RESEARCH|
Ministry of Human Resource Development (MHRD) under its National Mission on Education through Information and Communication Technology (NMEICT) has initiated the National Digital Library of India (NDL India) pilot project to develop a framework of virtual repository of learning resources with a single-window search facility. Filtered and federated searching is employed to facilitate focused searching so that learners can find out the right resource with least effort and in minimum time. NDL India is designed to hold content of any language and provides interface support for leading Indian languages. It is being arranged to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular form of access devices and differently-abled learners. It is being developed to help students to prepare for entrance and competitive examination, to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. The pilot project is devising a framework that is being scaled up with respect to content volume and diversity to serve all levels and disciplines of learners. It is being developed at Indian Institute of Technology Kharagpur.
NDL India is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with respective organization from where the contents are sourced and NDL India has no responsibility or liability for these. Every effort is made to keep the NDL India portal up and running smoothly. However, NDL India takes no responsibility for, and will not be liable for, the portal being unavailable due to technical issues or otherwise.
Ministry of Human Resource Development (MHRD), through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDL India) pilot project.
For any issue or feedback, please write to firstname.lastname@example.org
COVID-19 Lockdown not to affect your study. Study through National Digital Library of India (NDLI).