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Author John, Annie ♦ Varma, H. K. ♦ Vijayan, S. ♦ Bernhardt, A. ♦ Lode, A. ♦ Vogel, A. ♦ Burmeister, B. ♦ Hanke, Th ♦ Domaschke, H. ♦ Gelinsky, M.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Biomedical Materials (bristol, England)
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Domain (in MeSH) Biomedical and Dental Materials ♦ Chemicals and Drugs
Subject Keyword Biomaterials
Abstract The light microscopic examination of cells directly on bioceramic materials in the transmission mode is impossible because many of these materials are opaque. In order to enable direct viewing of living cells and to perform time-lapse studies, nearly transparent bioceramic materials were developed. A dense and fine-grained transparent hydroxyapatite (tHA) was processed by a gel-casting route followed by low-temperature sintering (1000 degrees C). By virtue of its transparency, direct visualization of cellular events on this material is possible in transmitted light. In this study, the interaction of different bone cell types with the tHA ceramic was envisaged. Investigation of rat calvaria osteoblasts (RCO) cultured on tHA by means of transmission light microscopy indicated good cytocompatibility of tHA. Microscopic analysis of osteogenic-induced human bone marrow stromal cells (hBMSC) on tHA and quantitative analysis of their lactate dehydrogenase (LDH) activity at different time points of culture revealed favorable proliferation as well. An increase of the alkaline phosphatase (ALP) activity indicated the differentiation of osteogenic-induced hBMSC towards the osteoblastic lineage. In addition, the differentiation of human monocytes to osteoclast-like cells could also be demonstrated on tHA and was confirmed by fluorescent microscopy imaging of multinucleated cells on the transparent material.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal Biomedical materials (Bristol, England)
Volume Number 4
Issue Number 1
Page Count 1
Starting Page 15007
Ending Page 15007