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Author Sailaja, G. S. ♦ Sreenivasan, K. ♦ Yokogawa, Y. ♦ Kumari, T. V. ♦ Varma, H. K.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Acta Biomaterialia
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Domain (in MeSH) Organic Chemicals ♦ Macromolecular Substances ♦ Biomedical and Dental Materials ♦ Chemicals and Drugs ♦ Cell Physiological Phenomena ♦ Biological Sciences
Subject Keyword Biocompatibility
Abstract Poly(vinyl alcohol) (PVA) films, when surface functionalized by phosphorylation, induced biomimetic nucleation and growth of calcium phosphate in a simulated physiological environment. The surface phosphorylation on PVA was ensured by attenuated total reflectance infrared spectroscopy. The morphology of the calcium phosphate phase grown on surface-phosphorylated PVA (PPVA) was analysed using scanning electron microscopy coupled with an energy-dispersive X-ray detector. The primary nucleation of calcium phosphate occurs in 3 days and secondary nucleation occurs after 10 days. The energy-dispersive X-ray analysis shows that the Ca/P ratio of the coating increases with time of exposure to the simulated physiological fluid and reaches 1.67 at 10 days. The PPVA supports in vitro cell adhesion and promotes in vitro biomineralization in the presence of cells, evaluated using human osteosarcoma cells. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal ACTA BIOMATERIALIA
Volume Number 5
Issue Number 5
Page Count 9
Starting Page 1647
Ending Page 1655