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Author Rathinam, K. ♦ Mohanan, P. V.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Journal of Biomaterials Applications
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Domain (in MeSH) Investigative Techniques ♦ Equipment and Supplies ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment
Subject Keyword Biological Evaluation
Abstract Medical devices of both intra- and extra-corporeal applications may contain leachable toxicants that warrant routine testing using internationally recognized/practiced protocols. An in vitro Microtox system comprising a selected bioluminescence strain of bacteria-Photobacterium phosphorium which emits light as an end product of its respiration, is used to screen out toxicants from biomaterials/devices. A Microtox system was standardized and validated in our laboratory conditions. Our experiences with extract of pyrogenic cardiotomy reservoir, blood filter and systemically toxic (in mice) Barium methacrylate (monomer), and CIBA epoxy polymer materials established a positive correlation with Microtox system. Hence, the Microtox system gives adequate evidence as an alternative in vitro method to evaluate the toxicity of biomaterials and medical devices.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Volume Number 13
Issue Number 2
Page Count 6
Starting Page 166
Ending Page 171