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Author Nair, L. V. ♦ Philips, D. S. ♦ Jayasree, R. S. ♦ Ajayaghosh, A.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Small
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Domain (in MeSH) Diagnosis ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment
Abstract The use of a nanosensor (AuC@Urease) based on an NIR-emitting gold cluster and urease enzyme selectively detects urea in whole blood. The detection is based on an enzyme-specific conversion of urea to ammonium ions which facilitates pH-induced aggregation of AuC, leading to fluorescence quenching. This method does not interfere with urease inactive analytes or the autofluorescence of blood as confirmed by comparable urea levels obtained by AuC@Urease and standard clinical methods within an error limit of ±3%
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal Small
Volume Number 2013