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Author Mohanan, P. V. ♦ Joseph, R. ♦ Ramesh, P. ♦ Rathinam, K.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Journal of Biomaterials Applications
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health ♦ Pharmacology and therapeutics
Subject Domain (in MeSH) Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Genetic Phenomena ♦ Biological Sciences
Subject Keyword Toxicology
Abstract Chromosomal aberrations are microscopically visible changes in the chromosome structure. The double-stranded breaks are the ultimate DNA lesions for chromosomal aberrations. The purpose of the present study was to evaluate the induction of chromosomal aberrations by the rubber accelerator zinc mercapto benzo thiazole (ZMBT). The experiment was designed with five groups, each composed of four Swiss albino mice. The first three groups received ZMBT at 1920, 960, and 483 mu g/20 g animal. The remaining two groups were the vehicle (cotton seed oil) and positive (methyl methane sulphonate) controls. Animals were given a single dose of test; and control samples by IP injection. Colchicine (20 mu g/animal) was administered 90 minutes before sacrificing the animals. All the animals were sacrificed at the end of 36 h by cervical dislocation. Bone marrow preparations were made, stained with Giemsa stain, and examined for chromosomal abnormalities. The results indicated a lack of incidence of chromosomal abnormalities in the test and control groups. However, significant chromosomal abnormalities such as gaps, breaks, and translocations were observed in the positive control group. Hence, the study concluded that ZMBT at different concentrations fails to induce structural chromosomal aberrations in bone marrow cells.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Volume Number 14
Issue Number 3
Page Count 5
Starting Page 224
Ending Page 228