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Author Pankajakshan, Divya ♦ Kalliyana, Krishnan V. ♦ Krishnan, L. K.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Biofabrication
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health ♦ Human physiology
Subject Domain (in MeSH) Tissues ♦ Cells ♦ Anatomy ♦ Biomedical and Dental Materials ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Equipment and Supplies ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment
Subject Keyword Tissue Engineering
Abstract Porous and pliable conduits made of biodegradable polymeric scaffolds offer great potential for the development of blood vessel substitutes but they generally lack signals for cell proliferation, survival and maintenance of a normal phenotype. In this study we have prepared and evaluated porous poly(epsilon-caprolactone) (PCL) integrated with fibrin composite (FC) to get a biomimetic hybrid scaffold (FC PCL) with the biological properties of fibrin, fibronectin (FN), gelatin, growth factors and glycosaminoglycans. Reduced platelet adhesion on a human umbilical vein endothelial cell-seeded hybrid scaffold as compared to bare PCL or FC PCL was observed, which suggests the non-thrombogenic nature of the tissue-engineered scaffold. Analysis of real-time polymerase chain reaction (RT-PCR) after 5 days of endothelial cell (EC) culture on a hybrid scaffold indicated that the prothrombotic von Willebrand factor and plasminogen activator inhibitor (PAI) were quiescent and stable. Meanwhile, dynamic expressions of tissue plasminogen activator (tPA) and endothelial nitric oxide synthase indicated the desired cell phenotype on the scaffold. On the hybrid scaffold, shear stress could induce enhanced nitric oxide release, which implicates vaso-responsiveness of EC grown on the tissue-engineered construct. Significant upregulation of mRNA for extracellular matrix (ECM) proteins, collagen IV and elastin, in EC was detected by RT-PCR after growing them on the hybrid scaffold and FC-coated tissue culture polystyrene (FC TCPS) but not on FN-coated TCPS. The results indicate that the FC PCL hybrid scaffold can accomplish a remodeled ECM and non-thrombogenic EC phenotype, and can be further investigated as a scaffold for cardiovascular tissue engineering.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal Biofabrication
Volume Number 2
Issue Number 4
Page Count 1
Starting Page 41001
Ending Page 41001