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Author Sailaja, G. S. ♦ Ramesh, P. ♦ Kumari, T. V. ♦ Varma, H. K.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Acta Biomaterialia
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Domain (in MeSH) Biomedical and Dental Materials ♦ Chemicals and Drugs ♦ Immune System Phenomena ♦ Biological Sciences
Subject Keyword Biocompatibility
Abstract A novel degradable composite system has been prepared by integrating hydroxyapatite, Ca-10(PO4)(6)(OH)(2), (HAP) in a polyelectrolyte complex matrix of chitosan (CHI) and poly(acrylic acid) (PAA). The composite was formulated by integrating 80 wt.% HAP in the polyelectrolyte complex matrix of CHI and PAA in the ratio 40/60 (designated as CPH). The composite could be easily fabricated into clinically significant shapes by a simple moulding procedure intended for bone graft applications. The adhesion behaviour of human osteosarcoma (HOS) cells on this degradable composite system was studied by selecting the polyelectrolyte complex, CHI/PAA 40/60 (designated as CP) as control sample. Light microscopic observations show that cells around CPH retained the typical morphology of HOS cells while cells around the polyelectrolyte complex showed a cytotoxic effect. The adhesion behaviour as well as morphological responses of the seeded cells was further investigated by scanning electron microscopy. The scanning electron micrographs of the polyelectrolyte complex, CP, showed the presence of rounded cells with raised nuclear regions, indicating delayed spreading; cells adhered on CPH were flattened with filopodia and showed good attachment and spreading, indicating better adhesion onto the HAP integrated composite. Comparing the MTT assay for quantitative evaluation of cell viability, CPH showed a higher percentage of metabolically active cells compared to CP. (c) 2006 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal ACTA BIOMATERIALIA
Volume Number 2
Issue Number 6
Page Count 7
Starting Page 651
Ending Page 657