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Author Arslan, P. ♦ Cantini, M. ♦ Cossarizza, A. ♦ Franceschi, C. ♦ Dall'acqua, F.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BASIC BIOLOGICAL SCIENCES ♦ RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT. ♦ COUMARIN ♦ BIOLOGICAL EFFECTS ♦ LYMPHOCYTES ♦ CELL PROLIFERATION ♦ DNA ADDUCTS ♦ IN VITRO ♦ MAN ♦ PSORALEN ♦ THYMIDINE ♦ TRACER TECHNIQUES ♦ TRITIUM COMPOUNDS ♦ ULTRAVIOLET RADIATION ♦ ADDUCTS ♦ ANIMAL CELLS ♦ ANIMALS ♦ ANTICOAGULANTS ♦ AZINES ♦ BIOLOGICAL MATERIALS ♦ BLOOD ♦ BLOOD CELLS ♦ BODY FLUIDS ♦ CONNECTIVE TISSUE CELLS ♦ COUMARINS ♦ DRUGS ♦ ELECTROMAGNETIC RADIATION ♦ ESTERS ♦ HEMATOLOGIC AGENTS ♦ HETEROCYCLIC COMPOUNDS ♦ ISOTOPE APPLICATIONS ♦ LABELLED COMPOUNDS ♦ LACTONES ♦ LEUKOCYTES ♦ MAMMALS ♦ MATERIALS ♦ NUCLEOSIDES ♦ NUCLEOTIDES ♦ ORGANIC COMPOUNDS ♦ ORGANIC NITROGEN COMPOUNDS ♦ ORGANIC OXYGEN COMPOUNDS ♦ PRIMATES ♦ PYRANS ♦ PYRIMIDINES ♦ RADIATIONS ♦ RIBOSIDES ♦ SOMATIC CELLS ♦ VERTEBRATES ♦ Biochemistry- Tracer Techniques ♦ Radiation Effects on Biochemicals, Cells, & Tissue Culture
Abstract The biological effects of three furocoumarins on the proliferation of human normal peripheral blood lymphocytes have been investigated. Mitogen-stimulated human lymphocytes were assayed in vitro by measuring /sup 3/H-thymidine (/sup 3/H-TdR) incorporation in the presence and in the absence of 15-30 /mu/M 3-carbethoxypsoralen (3-CPs), trimethylangelicin (TMA) and psoralen (PSR) with and without UV-A irradiation. The three furocoumarins differ in their ability to form mono- and bi-functional adducts with DNA pyrimidine furocoumarin doses and short times of UV-A irradiation used in the present study, 3-CPs did not affect /sup 3/H-TdR incorporation in PHA-stimulated human lymphocytes. TMA strongly inhibited /sup 3/H-TdR incorporation, while, unexpectedly, PSR increased /sup 3/H-TdR incorporation in the absence of irradiation, likely acting, under these experimental conditions, as a co-mitogen.
Educational Use Research
Learning Resource Type Article
Publisher Date 1989-01-01
Publisher Place United States
Journal Life Sci.
Volume Number 44
Issue Number 26
Organization Univ. of Padua (Italy)


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