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Author Nguyen, Long T. ♦ Rebecchi, Mario J. ♦ Moore, Leon C. ♦ Glass, Peter S. A. ♦ Brink, Peter R. ♦ Liu, Lixin
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Lippincott Williams & Wilkins
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Musculoskeletal System ♦ Cardiovascular System ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Inorganic Chemicals ♦ Organic Chemicals ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Therapeutics ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physical Phenomena ♦ Cell Physiological Phenomena ♦ Physiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Anesthesiology ♦ Anesthetics, Inhalation ♦ Pharmacology ♦ Heart ♦ Drug Effects ♦ Ischemic Preconditioning, Myocardial ♦ Isoflurane ♦ Myocardium ♦ Pathology ♦ Reactive Oxygen Species ♦ Aging ♦ Animals ♦ Cell Aging ♦ Male ♦ Models, Animal ♦ Models, Biological ♦ Rats ♦ Rats, Inbred F344 ♦ Time Factors ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract BACKGROUND: Although attenuation of anesthetic preconditioning in aged ex vivo heart models has been studied extensively, there are no comparable in vivo studies. To extend previous work and to address a possible mechanism underlying age-related differences, we investigated isoflurane-induced preconditioning and reactive oxygen species (ROS) production in the aged rat heart in vivo. METHODS: Male Fisher 344 rats were assigned from their respective age groups (young, 3-5 mo; old, 20-24 mo) to either receive 30 min of 1.0 minimum alveolar concentration isoflurane or to a control group. Rats were subjected to coronary artery occlusion for 30 min followed by 2 h of reperfusion. A fluorescent probe for superoxide anion production (dihydroethidium, 1 mg) was administered in the absence of the isoflurane or just before isoflurane exposure in four additional groups. Myocardial infarct size and superoxide anion production were assessed using triphenyltetrazolium staining and epifluorescence microscopy, respectively. RESULTS: Isoflurane decreased myocardial infarct size of young rats (26.7% +/- 3.0%) compared with young controls (50.9% +/- 1.9%; P < 0.001), whereas isoflurane did not significantly affect myocardial infarct size of old rats (39.1% +/- 0.9%) compared with old controls (46.5% +/- 2.4%; P > 0.05). Isoflurane increased ROS levels in young rats (430.5 +/- 95.9 arbitrary units [AU]) compared with young controls (162.7 +/- 25.5 AU; P < 0.01). In contrast, no significant changes in ROS levels were observed in old animals (316.4 +/- 56.3 AU isoflurane versus 233.8 +/- 59.2 AU control). CONCLUSIONS: Reduction in the cardioprotective effects of isoflurane and attenuation of isoflurane-stimulated ROS production were observed in the senescent myocardium in vivo.
Description Country affiliation: United States
Author Affiliation: Nguyen LT ( Department of Anesthesiology, Stony Brook University School of Medicine, HSC L4 060, Stony Brook, NY 11794, USA.)
ISSN 00032999
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2008-09-01
Publisher Place United States
e-ISSN 15267598
Journal Anesthesia & Analgesia
Volume Number 107
Issue Number 3

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Source: WHO-Global Index Medicus