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Author Imada, Yukio ♦ Yamada, Koichi
Source J-STAGE
Content type Text
Publisher Japan Society for Bioscience, Biotechnology, and Agrochemistry
Language English
Abstract As already reported, Corynebacterium hydrocarboclastus S10B1 was able to accumulate a good deal of L-glutamate in a thiamine-deficient medium at the sole expense of n-alkanes, but unable to form L-glutamate in a thiamine-sufficient medium though an abundant cell growth was observed. α-Ketoglutaric acid and DL-alanine were found to be produced in the same thiamine-deficient medium in which L-glutamate was accumulated. Both products formed from n-tetradecane by this organism were isolated from culture broth, purified and identified. The optimum concentration of thiamine in the culture medium was 3 to 5μg per liter for their production. The maximum yields of α-ketoglutaric acid and DL-alanine reached 16 g and 1.5g per liter in the calcium carbonate-added medium, respectively. However, the addition of more than 30μg per liter of thiamine extremely repressed their accumulation.
ISSN 00021369
Learning Resource Type Article
Publisher Date 1969-01-01
e-ISSN 18811280
Journal Agricultural and Biological Chemistry(bbb1961)
Volume Number 33
Issue Number 9
Page Count 7
Starting Page 1326
Ending Page 1332


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