Thumbnail
Access Restriction
Open

Author Kasahara, Hiroko ♦ Udaka, Shigezo ♦ Ikeda, Yonosuke
Source J-STAGE
Content type Text
Publisher Japan Society for Bioscience, Biotechnology, and Agrochemistry
Language English
Abstract The effectiveness of N-methyl-N'-nitro-N-nitrosoguanidine (NG) in inducing mutations in Streptomyces cacaoi was demonstrated with two systems: reversion to prototrophy of an isoleucine-requiring mutant and the induction of forward mutation of a prototrophic strain. An optimal condition for yielding a high frequency mutation was set as follows: Treat-ment of spores with 1mg/ml of NG in 0.016M phosphate buffer, pH 6, at 42°C for 60min. In Streptmyces cacaoi, the mutagenesis depended highly on the temperature of NG treatment; elevation of the treatment temperature resulted higher frequency of mutation. Only a little dependency on temperature was shown in Escherichia coli, but the dependency was also observed when the Streptomyces was treated with ethyl methanesulfonate. The significance of these results for considering mechanism of NG action was discussed.
ISSN 00021369
Learning Resource Type Article
Publisher Date 1971-01-01
e-ISSN 18811280
Journal Agricultural and Biological Chemistry(bbb1961)
Volume Number 35
Issue Number 2
Page Count 7
Starting Page 226
Ending Page 232


Open content in new tab

   Open content in new tab