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Author Henson, P. ♦ Selsky, C. A. ♦ Little, J. B.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT. ♦ ENVIRONMENTAL SCIENCES ♦ DNA ♦ BIOLOGICAL REPAIR ♦ FIBROBLASTS ♦ GENETIC RADIATION EFFECTS ♦ CAFFEINE ♦ CELL CULTURES ♦ COMPARATIVE EVALUATIONS ♦ DIMERS ♦ DNA REPLICATION ♦ MAN ♦ METABOLISM ♦ PYRIMIDINES ♦ TELANGIECTASIS ♦ ULTRAVIOLET RADIATION ♦ ANALEPTICS ♦ ANIMAL CELLS ♦ ANIMALS ♦ AZINES ♦ BIOLOGICAL EFFECTS ♦ BIOLOGICAL RADIATION EFFECTS ♦ BIOLOGICAL RECOVERY ♦ CENTRAL NERVOUS SYSTEM AGENTS ♦ CONNECTIVE TISSUE CELLS ♦ DISEASES ♦ DRUGS ♦ ELECTROMAGNETIC RADIATION ♦ GENETIC EFFECTS ♦ HETEROCYCLIC COMPOUNDS ♦ MAMMALS ♦ NUCLEIC ACID REPLICATION ♦ NUCLEIC ACIDS ♦ ORGANIC COMPOUNDS ♦ ORGANIC NITROGEN COMPOUNDS ♦ ORGANIC OXYGEN COMPOUNDS ♦ PRIMATES ♦ PURINES ♦ RADIATION EFFECTS ♦ RADIATIONS ♦ RECOVERY ♦ REPAIR ♦ SKIN DISEASES ♦ SOMATIC CELLS ♦ VASCULAR DISEASES ♦ VERTEBRATES ♦ XANTHINES ♦ Radiation Effects on Cells- External Source- (-1987) ♦ Environment, Atmospheric- Radioactive Materials Monitoring & Transport- (-1989) ♦ Environment, Atmospheric- Chemicals Monitoring & Transport- (-1989)
Abstract Researchers have studied repair of ultraviolet light-induced damage in a strain of Bloom's syndrome cells which we have shown to be defective in host cell reactivation of uv-irradiated herpes simplex virus. Excision repair was monitored by following loss of sensitivity of DNA in permeabilized cells to digestion by the Micrococcus luteus uv endonuclease preparation. The Bloom's syndrome fibroblasts apparently removed endonuclease-sensitive sites from the DNA slightly less efficiently than did normal strains. After 24 h, 38% of the sites remained in the Bloom's syndrome cells in comparison with 16% in normal fibroblasts. DNA newly synthesized in uv-irradiated Bloom's syndrome cells sedimented less far into alkaline sucrose gradients than did DNA from similarly treated normal cells. In other respects, including the effect of caffeine exposure, DNA synthesis in Bloom's syndrome cells was indistinguishable from that in normal cells. We were therefore able to detect only minor defects in the repair of uv-induced damage in Bloom's syndrome fibroblasts. This is consistent with the normal survival exhibited by these cells. The defect in excision repair may, however, be sufficient to allow the cellular repair capacity to become saturated at high infecting multiplicities of uv-irradiated herpes simplex virus.
Educational Use Research
Learning Resource Type Article
Publisher Date 1981-03-01
Publisher Place United States
Journal Cancer Res.
Volume Number 41
Issue Number 3


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