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Author Lee, Soo-Ho ♦ Kim, Chowon ♦ Lee, Hyun-Kyung ♦ Kim, Yoo-Kyung ♦ Ismail, Tayaba ♦ Jeong, Youngeun ♦ Park, Kyungyeon ♦ Park, Mae-Ja ♦ Park, Do-Sim ♦ Lee, Hyun-Shik
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ INDUCTION ♦ ONTOGENESIS ♦ PLANT GROWTH
Abstract NSrp70 (nuclear speckle-related protein 70), a recently discovered protein and it belongs to the serine/arginine (SR) rich related protein family. NSrp70 is recognized as an important splicing factor comprising RNA recognition motif (RRM) and arginine/serine (RS)-like regions at the N- and C-terminus respectively, along with two coiled coil domains at each terminus. However, other functions of NSrp70 remain unelucidated. In this study, we investigated the role of NSrp70 in Xenopus embryogenesis and found that its maternal expression plays a critical role in embryonic development. Knockdown of NSrp70 resulted in dramatic reduction in the length of developing tadpoles and mild to severe malformation in Xenopus embryos. In addition, knockdown of NSrp70 resulted in an extremely short axis by blocking gastrulation and convergent extension. Further, animal cap assays along with activin A treatment revealed that NSrp70 is an essential factor for dorsal mesoderm induction as knockdown of NSrp70 caused a dramatic down-regulation of dorsal mesoderm specific genes and its loss significantly shortened the elongation region of animal caps. In conclusion, NSrp70 is crucial for early embryonic development, influencing gastrulation and mesoderm induction. - Highlights: • NSrp70 is required for proper length and dorso-anterior structures. • NSrp70 is essential for gastrulation movement during embryogenesis. • NSrp70 is crucial component for dorsal mesoderm induction.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-10-14
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 479
Issue Number 2


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