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Author Lerch, Jason P. ♦ Kushner, Steven A. ♦ Derek, Van Der Kooy ♦ Frankland, Paul W. ♦ Leslie, Ana T. ♦ Laura, Clarke ♦ Akers, Katherine G.
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher BioMed Central
File Format HTM / HTML
Date Created 2013-03-12
Copyright Year ©2011
Language English
Subject Domain (in LCC) RC346-429
Subject Keyword Biological psychiatry ♦ Neurosciences ♦ Olfactory bulb ♦ MRI ♦ Internal medicine ♦ Medicine ♦ Odor discrimination ♦ Subependymal zone ♦ Neuropsychiatry ♦ Fetal alcohol exposure ♦ Diseases of the nervous system ♦ Neurogenesis ♦ Neurospheres ♦ Odor memory ♦ Neurology
Abstract Abstract Background Children whose mothers consumed alcohol during pregnancy exhibit widespread brain abnormalities and a complex array of behavioral disturbances. Here, we used a mouse model of fetal alcohol exposure to investigate relationships between brain abnormalities and specific behavioral alterations during adulthood. Results Mice drank a 10% ethanol solution throughout pregnancy. When fetal alcohol-exposed offspring reached adulthood, we used high resolution MRI to conduct a brain-wide screen for structural changes and found that the largest reduction in volume occurred in the olfactory bulbs. Next, we tested adult mice in an associative olfactory task and found that fetal alcohol exposure impaired discrimination between similar odors but left odor memory intact. Finally, we investigated olfactory bulb neurogenesis as a potential mechanism by performing an in vitro neurosphere assay, in vivo labeling of new cells using BrdU, and in vivo labeling of new cells using a transgenic reporter system. We found that fetal alcohol exposure decreased the number of neural precursor cells in the subependymal zone and the number of new cells in the olfactory bulbs during the first few postnatal weeks. Conclusions Using a combination of techniques, including structural brain imaging, in vitro and in vivo cell detection methods, and behavioral testing, we found that fetal alcohol exposure results in smaller olfactory bulbs and impairments in odor discrimination that persist into adulthood. Furthermore, we found that these abnormalities in olfactory bulb structure and function may arise from deficits in the generation of new olfactory bulb neurons during early postnatal development.
ISSN 17566606
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2011-07-01
e-ISSN 17566606
Journal Molecular Brain
Volume Number 4
Issue Number 1
Starting Page 29


Source: Directory of Open Access Journals (DOAJ)