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Author Kanno, Ayumi ♦ Asahara, Shun-ichiro ♦ Masuda, Katsuhisa ♦ Matsuda, Tomokazu ♦ Kimura-Koyanagi, Maki ♦ Seino, Susumu ♦ Ogawa, Wataru ♦ Kido, Yoshiaki
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ CARBOXYLIC ACIDS ♦ CORRELATIONS ♦ CYCLOHEXIMIDE ♦ DIABETES MELLITUS ♦ DIET ♦ FATS ♦ GLUCOSE ♦ HYDROFLUORIC ACID ♦ HYPERGLYCEMIA ♦ INSULIN ♦ INTAKE ♦ MICE ♦ ONCOGENES ♦ PANCREAS ♦ SECRETION ♦ SYNTHESIS ♦ TOLERANCE ♦ TRANSCRIPTION
Abstract A high-fat diet (HF) is associated with obesity, insulin resistance, and hyperglycemia. Animal studies have shown compensatory mechanisms in pancreatic β-cells after high fat load, such as increased pancreatic β-cell mass, enhanced insulin secretion, and exocytosis. However, the effects of high fat intake on insulin synthesis are obscure. Here, we investigated whether insulin synthesis was altered in correlation with an HF diet, for the purpose of obtaining further understanding of the compensatory mechanisms in pancreatic β-cells. Mice fed an HF diet are obese, insulin resistant, hyperinsulinemic, and glucose intolerant. In islets of mice fed an HF diet, more storage of insulin was identified. We analyzed insulin translation in mouse islets, as well as in INS-1 cells, using non-radioisotope chemicals. We found that insulin translational levels were significantly increased in islets of mice fed an HF diet to meet systemic demand, without altering its transcriptional levels. Our data showed that not only increased pancreatic β-cell mass and insulin secretion but also elevated insulin translation is the major compensatory mechanism of pancreatic β-cells. - Highlights: • More stored insulin was recognized in islets of mice fed a high-fat diet. • Insulin translation was not enhanced by fatty acids, but by insulin demand. • Insulin transcription was not altered in islets of mice fed a high-fat diet. • Insulin translation was markedly enhanced in islets of mice fed a high-fat diet. • Non-radioisotope chemicals were used to measure insulin translation in mouse islets.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-03-13
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 458
Issue Number 3


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