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Author Xu, Chengming ♦ Ruan, Banjun ♦ Jiang, Yinghao ♦ Xue, Ting ♦ Wang, Zhenyu ♦ Lu, Huanyu ♦ Wei, Ming ♦ Wang, Shan ♦ Ye, Zicheng ♦ Zhai, Dongsheng ♦ Wang, Li ♦ Lu, Zifan
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ANTIBIOTICS ♦ ANTIGENS ♦ CELL PROLIFERATION ♦ HOMEOSTASIS ♦ INSPECTION ♦ LYMPHOKINES ♦ MEDICAL SURVEILLANCE ♦ MELANOMAS ♦ MICE ♦ ORAL ADMINISTRATION ♦ PLANT GROWTH ♦ SECRETION
Abstract Gut microbiota is critical for maintaining body immune homeostasis and thus affects tumor growth and therapeutic efficiency. Here, we investigated the link between microbiota and tumorgenesis in a mice model of subcutaneous melanoma cell transplantation, and explored the underlying mechanism. We found disruption of gut microbiota by pretreating mice with antibiotics promote tumor growth and remodeling the immune compartment within the primary tumor. Indeed, gut microbial dysbiosis reduced the infiltrated mature antigen-presenting cells of tumor, together with lower levels of co-stimulators, such as CD80, CD86 and MHCII, as well as defective Th1 cytokines, including IFNγ, TNFα, IL12p40, and IL12p35. Meantime, splenic APCs displayed blunted ability in triggering T cell proliferation and IFNγ secretion. However, oral administration of LPS restored the immune surveillance effects and thus inhibited tumor growth in the antibiotics induced gut microbiota dysbiosis group. Taken together, these data highly supported that antibiotics induced gut microbiota dysbiosis promotes tumor initiation, while LPS supplementation would restore the effective immune surveillance and repress tumor initiation. - Highlights: • Antibiotics induced gut microbiota dysbiosis promotes tumor initiation in mice. • Gut microbiota disruption compromise APC-Th1 development in tumor. • Orally LPS administration rescues tumor immune surveillance in gut microbiota disrupted mice. • None of the authors have any conflict of interest.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2017-06-24
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 488
Issue Number 2


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