|Author||Takahashi, Kazutoshi ♦ Ogawa, Tetsuhiro ♦ Hidaka, Makoto ♦ Ohsawa, Kanju ♦ Masaki, Haruhiko ♦ Yajima, Shunsuke|
|Source||World Health Organization (WHO)-Global Index Medicus|
|File Format||HTM / HTML|
|Subject Domain (in DDC)||Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Crystallography ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices|
|Subject Domain (in MeSH)||Bacteria ♦ Organisms ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Metabolism ♦ Biological Sciences|
|Subject Keyword||Discipline Crystallography ♦ Discipline Biophysics ♦ Discipline Molecular Biology ♦ Discipline Biochemistry ♦ Catalytic Domain ♦ Endoribonucleases ♦ Chemistry ♦ Escherichia Coli Proteins ♦ Escherichia Coli ♦ Enzymology ♦ Crystallization ♦ Crystallography, X-ray ♦ Metabolism ♦ Protein Transport ♦ Rna, Bacterial ♦ Rna, Transfer, Amino Acyl ♦ Journal Article|
|Abstract||The tRNase domain of colicin D, which cleaves only tRNA(Arg)s at the 3' side of their anticodon loops, has been expressed in Escherichia coli with its inhibitor protein and purified to a form free from the inhibitor using a low-pH buffer. Crystals were obtained by the hanging-drop vapour-diffusion method at 278 K from a buffer containing 100 mM Tris-HCl pH 8.5, 22% PEG MME 2000 and 1 mM nickel(II) chloride. Diffraction data to 1.05 A resolution were collected at BL41XU, SPring-8. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.7, b = 65.5, c = 96.5 A.|
|Description||Country affiliation: Japan
Author Affiliation: Takahashi K ( Department of Biotechnology, The University of Tokyo, Yayoi 1-1-1, Tokyo 113-8657, Japan.)
|Educational Role||Student ♦ Teacher|
|Age Range||above 22 year|
|Educational Use||Reading ♦ Research ♦ Self Learning|
|Education Level||UG and PG|
|Learning Resource Type||Article|
|Publisher Place||Great Britain (UK)|
|Journal||Acta Crystallographica Section F Structural Biology and Crystallization Communications|
|Issue Number||Pt 1|
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