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Author Tylichová, Martina ♦ Briozzo, Pierre ♦ Kopecný, David ♦ Ferrero, Julien ♦ Moréra, Solange ♦ Joly, Nathalie ♦ Snégaroff, Jacques ♦ Sebela, Marek
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Crystallography ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Eukaryota ♦ Organisms ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Biological Sciences
Subject Keyword Discipline Crystallography ♦ Discipline Biophysics ♦ Discipline Molecular Biology ♦ Discipline Biochemistry ♦ Aldehyde Dehydrogenase ♦ Chemistry ♦ Peas ♦ Enzymology ♦ Isolation & Purification ♦ Metabolism ♦ Base Sequence ♦ Blotting, Western ♦ Cloning, Molecular ♦ Crystallization ♦ Crystallography, X-ray ♦ Dna Primers ♦ Electrophoresis, Polyacrylamide Gel ♦ Protein Conformation ♦ Recombinant Proteins ♦ Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Aminoaldehydes are products of polyamine degradation and are known to be reactive metabolites that are toxic to living cells at high concentrations. These compounds are catabolized by aminoaldehyde dehydrogenases, which are enzymes that contain a nicotinamide adenine dinucleotide coenzyme. Aminoaldehyde dehydrogenase from Pisum sativum was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop method. A complete data set was collected to 2.8 A resolution at 100 K. Crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 86.4, b = 216.6, c = 205.4 A, beta = 98.1 degrees. Molecular replacement was performed and led to the identification of six dimers per asymmetric unit.
Description Country affiliation: Czech Republic
Author Affiliation: Tylichová M ( Department of Biochemistry, Faculty of Science, Palacký University, Slechtitelu 11, CZ-78371 Olomouc, Czech Republic.)
ISSN 2053230X
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2008-02-01
Publisher Place Great Britain (UK)
e-ISSN 17443091
Journal Acta Crystallographica Section F Structural Biology and Crystallization Communications
Volume Number 64
Issue Number Pt 2

Source: WHO-Global Index Medicus