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Author Balamuralikrishnan, M. ♦ Viswanathan, R.
Source SpringerLink
Content type Text
Publisher Springer India
File Format PDF
Copyright Year ©1999
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Sugarcane bacilliform virus ♦ PCR ♦ Dac-Elisa ♦ molecular diagnosis ♦ Agriculture
Abstract The polymerase chain reaction (PCR) and direct antigen coating enzyme linked immunosorbant assay (DAC-ELISA) were compared for the diagnosis of sugarcane bacilliform virus (SCBV) in sugarcane clones. The SCBV specific primers were used for PCR and polyclonal antiserum for SCBV was used in DAC-ELISA. Eleven clones suspected for SCBV foliar symptoms comprising of different species of Saccharum, interspecific hybrids and allied genera were taken and an apparently healthy clone was also taken for the comparison. In PCR a 221bp DNA fragment was amplified in all the eleven clones with suspected foliar symptoms and there was no amplification in the apparently healthy clone. In DAC-ELISA a positive reaction was obtained only in eight SCBV suspected clones indicating higher sensitivity of PCR for the diagnosis of SCBV and it could be effectively used during quarantine operations for the sugarcane germplasm exchange.
ISSN 09721525
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 1999-01-01
Publisher Place New Delhi
e-ISSN 09740740
Journal Sugar Tech
Volume Number 7
Issue Number 4
Page Count 4
Starting Page 119
Ending Page 122

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Source: SpringerLink