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Author Muto, Nozomu ♦ Takeshima, Hirohiko ♦ Kakioka, Ryo ♦ Alama, Ulysses B. ♦ Guzman, Armi May T. ♦ Cruz, Ramon S. ♦ Gaje, Arnold C. ♦ Traifalgar, Rex Ferdinand M. ♦ Motomura, Hiroyuki ♦ Muto, Fumihito ♦ Babaran, Ricardo P. ♦ Ishikawa, Satoshi
Source SpringerLink
Content type Text
Publisher Springer Berlin Heidelberg
File Format PDF
Copyright Year ©2016
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Mackerel ♦ Rastrelliger ♦ PCR-RFLP ♦ mtDNA ♦ Biodiversity ♦ Freshwater & Marine Ecology ♦ Plant Systematics/Taxonomy/Biogeography ♦ Animal Systematics/Taxonomy/ Biogeography
Abstract The three mackerel species, Rastrelliger kanagurta, Rastrelliger brachysoma, and Rastrelliger faughni, co-occur in the central part of the Indo-West Pacific Ocean, and together represent important fisheries resources. However, they have sometimes been collectively referred to as “Rastrelliger spp.” in the fisheries statistics, owing to their morphological similarity. Therefore, species-specific data on landings, effort, and population status are lacking, undermining the appropriate resource management. The present study provides a rapid and cost-effective method based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis to distinguish the three species. Digestion of the PCR products of 1247 base pairs of mitochondrial DNA encompassing the complete sequence of cytochrome b gene using two restriction enzymes yielded diagnostic restriction patterns for the three species, thereby enabling accurate identification.
ISSN 18671616
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2016-07-05
Publisher Place Berlin, Heidelberg
e-ISSN 18671624
Journal Marine Biodiversity
Volume Number 47
Issue Number 2
Page Count 3
Starting Page 609
Ending Page 611


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Source: SpringerLink