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Author Sato, Younosuke ♦ Matsuo, Akira ♦ Kudoh, Shinji ♦ Fang, Liu ♦ Hasegawa, Koki ♦ Shinmyo, Yohei ♦ Ito, Takaaki
Source J-STAGE
Content type Text
Publisher JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
Language English
Subject Keyword lung cancer ♦ Draxin ♦ Netrin-1 ♦ dependence receptor ♦ cell proliferation
Abstract Guidance molecules, such as Netrin-1, and their receptors have important roles in controlling axon pathfinding, modulate biological activities of various cancer cells, and may be a useful target for cancer therapy. Dorsal repulsive axon guidance protein (Draxin) is a novel guidance molecule that binds not only common guidance molecule receptors with Netrin-1, but also directly binds the EGF domain of Netrin-1 through a 22-amino-acid peptide (22aa). By immunostaining, Draxin was positively expressed in small cell carcinoma, adenocarcinoma (ADC), and squamous cell carcinoma of the lung. In addition, western blot analysis revealed that Draxin was expressed in all histological types of lung cancer cell lines examined. Knockdown of Draxin in an ADC cell line H358 resulted in altered expression of molecules associated with proliferation and apoptosis. The Ki-67 labeling index of Draxin-knockdown ADC cells was increased compared to that of control ADC cells. In H358 cells, treatment of 22aa induced phosphorylation of histone H3, but did not change apoptosis-associated enzymes. These data suggest that Draxin might be involved in cell proliferation and apoptosis in lung adenocarcinoma cells.
ISSN 00445991
Learning Resource Type Article
Publisher Date 2018-02-27
e-ISSN 13475800
Journal ACTA HISTOCHEMICA ET CYTOCHEMICA(ahc)
Volume Number 51
Issue Number 1
Page Count 10
Starting Page 53
Ending Page 62


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