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Author MaruYama, Takashi
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ANTIBODIES ♦ CONTROL ♦ ENZYMES ♦ GENE REGULATION ♦ GENES ♦ INTERFERON ♦ POLYMERASE CHAIN REACTION ♦ PROMOTERS ♦ RECEPTORS ♦ SIGNALS
Abstract Inhibitor of kappa B (IκB)-ζ, a member of the nuclear IκB family of proteins, is induced by the transforming growth factor (TGF)-β signaling pathway and plays a pivotal role in maintaining the balance of T helper (Th) cell subsets. IκB-ζ deficiency results in reduced percentages of Th17 cells and increased percentages of Th1 cells. In this study, the effects of IκB-ζ deficiency on T-cell subsets were examined further. The data showed that IκB-ζ-deficient T cells had a high capacity for generation of regulatory T cells (Tregs) when T cells were cultured under TGF-β stimulation in the presence of cytokine-neutralizing antibodies. Mechanistically, IκB-ζ itself negatively regulated activation of the Foxp3 promoter in a nuclear factor of kappaB-dependent manner. Thus, this study showed that IκB-ζ controlled Treg differentiation. - Highlights: • IκB-ζ-deficient T cells exhibited increased generation of Foxp3{sup +} Tregs. • IκB-ζ played a key role in Foxp3 gene expression. • Retroviral overexpression of IκB-ζ was achieved in T cells.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-08-21
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 464
Issue Number 2


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