Thumbnail
Access Restriction
Open

Author Stratton, Dan ♦ Moore, Colin ♦ Antwi-Baffour, Samuel ♦ Lange, Sigrun ♦ Inal, Jameel
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ CALCIUM ♦ COMPARATIVE EVALUATIONS ♦ COMPLEMENT ♦ DEPOSITS ♦ INHIBITION ♦ LIGHT SCATTERING ♦ LIPIDS ♦ METABOLISM ♦ MICROBALANCES ♦ NEOPLASMS ♦ PROSTATE ♦ QUARTZ ♦ SENSORS ♦ STIMULATION ♦ SURFACES
Abstract We have classified microvesicles into two subtypes: larger MVs released upon stimulation of prostate cancer cells, sMVs, and smaller cMVs, released constitutively. cMVs are released as part of cell metabolism and sMVs, released at 10-fold higher levels, produced upon activation, including sublytic C5b-9. From electron microscopy, nanosight tracking analysis, dynamic light scattering and flow cytometry, cMVs (194–210 nm in diameter) are smaller than sMVs (333–385 nm). Furthermore, using a Quartz Crystal Microbalance measuring changes in resonant frequency (Δf) that equate to mass deposited on a sensor, an sMV and a cMV are estimated at 0.267 and 0.241 pg, respectively. sMVs carry more calcium and protein, express higher levels of lipid rafts, GPI-anchored CD55 and phosphatidylserine including deposited C5b-9 compared to cMVs. This may allude to biological differences such as increased bound C4BP on sMVs inhibiting complement more effectively. - Highlights: • Prostate cells release microvesicles constitutively (cMVs) or upon stimulus (sMVs). • sMVs are larger than cMVs and carry more protein, lipid rafts and surface PstSer. • sMVs inhibit complement more effectively than cMVs.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-05-08
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 460
Issue Number 3


Open content in new tab

   Open content in new tab