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Author Yao, Jingjing ♦ Xu, Chen ♦ Fang, Ziyu ♦ Li, Yaoming ♦ Liu, Houqi ♦ Wang, Yue ♦ Xu, Chuanliang ♦ Sun, Yinghao
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ANDROGENS ♦ ANIMAL TISSUES ♦ APOPTOSIS ♦ CELL PROLIFERATION ♦ CHINA ♦ DIAGNOSIS ♦ GENES ♦ IN VITRO ♦ IN VIVO ♦ MESSENGER-RNA ♦ METASTASES ♦ NEOPLASMS ♦ PATIENTS ♦ PLANT TISSUES ♦ POLYMERASE CHAIN REACTION ♦ PROSTATE ♦ RECEPTORS ♦ TRANSCRIPTION
Abstract Abstracts: MicroRNAs (miRNAs) are important endogenous gene regulators that play key roles in prostate cancer development and metastasis. However, specific miRNA expression patterns in prostate cancer tissues from Chinese patients remain largely unknown. In this study, we compared miRNA expression patterns in 65 pairs of prostate cancer and para-cancer tissues by RNA sequencing and found that miR-182-5p was the most up-regulated miRNA in prostate cancer tissues. The result was validated using realtime PCR in 18 pairs of prostate cancer and para-cancer tissues. In in vitro analysis, it was confirmed that miR-182-5p promotes prostate cancer cell proliferation, invasion and migration and inhibit apoptosis. In addition, the androgen receptor directly regulated the transcription of miR-182-5p, which could target to the 3′UTR of ARRDC3 mRNA and affect the expression of ARRDC3 and its downstream gene ITGB4. For the in vivo experiment, miR-182-5p overexpression also promoted the growth and progression of prostate cancer tumors. In this regard, we suggest that miR-182-5p may be a key androgen receptor-regulated factor that contributes to the development and metastasis of Chinese prostate cancers and may be a potential target for the early diagnosis and therapeutic studies of prostate cancer. -- Highlights: •miR-182-5p is the mostly up-regulated miRNA in Chinese prostate cancer. •miR-182-5p is regulated by androgen receptor. •miR-182-5p promotes prostate cancer progression. •miR-182-5p regulates ARRDC3/ITGB4 pathway.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-05-20
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 474
Issue Number 1


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