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Author Jackson, M. S. ♦ Lauffart, B. F. ♦ See, Chee Gee ♦ Mulligan, L. M.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BIOLOGY AND MEDICINE, BASIC STUDIES ♦ HUMAN CHROMOSOMES ♦ DNA SEQUENCING ♦ GENETIC MAPPING ♦ CHROMOSOMAL ABERRATIONS ♦ STRUCTURE-ACTIVITY RELATIONSHIPS ♦ DNA-CLONING ♦ PROTEINS ♦ TRANSCRIPTION ♦ PRIMATES ♦ BIOLOGICAL EVOLUTION ♦ CONTIGS ♦ BIOLOGICAL MARKERS ♦ PROBES ♦ FLUORESCENCE ♦ POLYMERASE CHAIN REACTION ♦ IN-SITU HYBRIDIZATION ♦ ELECTROPHORESIS ♦ CENTROMERES
Abstract We present a yeast artificial chromosome (YAC) and pulsed-field gel electrophoresis (PFGE) map across the centromere of human chromosome 10 that links expressed sequences in 10p11 to expressed sequences in 10q11.2. This map is the first of its kind to link genes across a human centromere. It consists of a 2.5-Mb YAC contig extending from 10p11 to our previously published 5.35-Mb YAC contig extending from these satellite arrays to 10q11.2. This map covers approximately 6.5-7% of the total DNA of chromosome 10. Two Genethon genetic markers, D10S578 and D10S604, are included. These markers are only 1 cM apart but are separated by a physical distance of more than 9.2 Mb, including the centromere. This gives a ratio of genetic to physical distance of 0.11 cM/Mb, 9-11 times lower than average estimates for the human genome and chromosome 10. Markers linked to the centromere include the duplicated zinc finger genes ZNF11A, ZNF33A, and ZNF37A (which map to 10p11) and ZNF11B, ZNF33B, and ZNF37B (which map to 10q11.2). Restriction mapping confirms that the genes on each are lie in opposite orientation with respect to the centromere, consistent with the hypothesis that a pericentric inversion has occurred in this region during primate evolution. 68 refs., 6 figs., 2 tabs.
ISSN 08887543
Educational Use Research
Learning Resource Type Article
Publisher Date 1996-04-15
Publisher Place United States
Journal Genomics
Volume Number 33
Issue Number 2


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