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Author Pilotto, F. ♦ Santos, Lr Dos ♦ Mion, L. ♦ Parizotto, L. ♦ Nascimento, Vp Do ♦ Rodrigues, Lb ♦ Colla, Fl ♦ Dickel, El
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher Fundação APINCO de Ciência e Tecnologia Avícolas
File Format HTM / HTML
Date Created 2015-12-15
Copyright Year ©2014
Language English
Subject Domain (in LCC) SF1-1100 ♦ SF600-1100 ♦ QL1-991
Subject Keyword Agriculture ♦ Salmonella ♦ Chicken meat ♦ Science ♦ Animal culture ♦ Veterinary medicine ♦ Most probable number ♦ Zoology
Abstract Salmonella is traditionally identified by conventional microbiological tests, but the enumeration of this bacterium is not used on a routine basis. Methods such as the most probable number (MPN), which utilize an array of multiple tubes, are time-consuming and expensive, whereas miniaturized most probable number (mMPN) methods, which use microplates, can be adapted for the enumeration of bacteria, saving up time and materials. The aim of the present paper is to assess two mMPN methods for the enumeration of Salmonella sp in artificially-contaminated chicken meat samples. Microplates containing 24 wells (method A) and 96 wells (method B), both with peptone water as pre-enrichment medium and modified semi-solid Rappaport-Vassiliadis (MSRV) as selective enrichment medium, were used. The meat matrix consisted of 25g of autoclaved ground chicken breast contaminated with dilutions of up to 10(6) of Salmonella Typhimurium (ST) and Escherichia coli (EC). In method A, the dilution 10-5 of Salmonella Typhimurium corresponded to >57 MPN/mL and the dilution 10-6 was equal to 30 MPN/mL. There was a correlation between the counts used for the artificial contamination of the samples and those recovered by mMPN, indicating that the method A was sensitive for the enumeration of different levels of contamination of the meat matrix. In method B, there was no correlation between the inoculated dilutions and the mMPN results.
ISSN 1516635X
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2014-03-01
e-ISSN 1516635X
Journal Brazilian Journal of Poultry Science
Volume Number 16
Issue Number 1
Page Count 4
Starting Page 45
Ending Page 48

Source: Directory of Open Access Journals (DOAJ)