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Author Soares, Michelle ♦ Saussoy, Pascale ♦ Sahrari, Karima ♦ Amorim, Christiani A. ♦ Donnez, Jacques ♦ Dolmans, Marie-Madeleine
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Springer
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Diseases ♦ Surgery & related medical specialties ♦ Gynecology, obstetrics, pediatrics & geriatrics ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Urogenital System ♦ Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Neoplasms ♦ Diseases ♦ Surgical Procedures, Operative ♦ Investigative Techniques ♦ Equipment and Supplies ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment
Subject Keyword Discipline Reproductive Medicine ♦ Discipline Genectics ♦ Artificial Organs ♦ Leukemia ♦ Pathology ♦ Models, Biological ♦ Neoplasm Transplantation ♦ Methods ♦ Ovary ♦ Animals ♦ Female ♦ Humans ♦ Mice ♦ Mice, Scid ♦ Stromal Cells ♦ Transplantation ♦ Transplantation, Heterologous ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract PURPOSE: To evaluate the tumor-inducing ability of a few leukemic cells xenotransplanted inside an artificial ovary. METHODS: Ten and 100 BV-173 leukemic cells were embedded in a fibrin matrix along with 50,000 human ovarian stromal cells, and grafted to the peritoneal bursa of 5 and 5 SCID mice respectively. Four mice grafted with 3x10(6) leukemic cells in fibrin served as positive controls. At 20 weeks post-transplantation, the grafts, liver, spleen, blood and bone marrow were analyzed for the presence of leukemia by anti-CD79 IHC, flow cytometry (FC) and PCR. RESULTS: All mice grafted with 3x10(6) cells developed peritoneal masses 4 weeks after xenotransplantation, and systemic disease was confirmed by IHC, PCR and FC. Among mice grafted with 10 or 100 leukemic cells, none showed any sign of leukemia after 20 weeks, and IHC, FC and PCR on the different recovered tissues all proved negative. CONCLUSION: This study investigates the tumor-inducing potential of a few leukemic cells grafted inside an artificial ovary. Transplantation of 100 leukemic cells appears to be insufficient to induce leukemia after 20 weeks. These results in an immunodeficient xenografting model are quite reassuring. However, for clinical application, follicle suspensions must be purged of leukemic cells before grafting, as even the slightest risk should be avoided.
Description Country affiliation: Belgium
Author Affiliation: Soares M ( Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.)
ISSN 10580468
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2015-04-01
Publisher Place Netherlands
e-ISSN 15737330
Journal Journal of Assisted Reproduction and Genetics
Volume Number 32
Issue Number 4

Source: WHO-Global Index Medicus