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Author Bedaiwy, Mohamed A. ♦ Falcone, Tommaso ♦ Goldberg, Jeffrey M. ♦ Attaran, Marjan ♦ Sharma, Rakesh ♦ Miller, Kurt ♦ Nelson, David R. ♦ Agarwal, Ashok
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Springer
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Gynecology, obstetrics, pediatrics & geriatrics ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Urogenital System ♦ Fluids and Secretions ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Female Urogenital Diseases and Pregnancy Complications ♦ Diseases ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physiological Phenomena ♦ Reproductive and Urinary Physiological Phenomena ♦ Biological Sciences ♦ Persons ♦ Persons
Subject Keyword Discipline Reproductive Medicine ♦ Discipline Genectics ♦ Cytokines ♦ Physiology ♦ Embryo Loss ♦ Etiology ♦ Embryonic Development ♦ Fallopian Tubes ♦ Adult ♦ Animals ♦ Body Fluids ♦ Immunology ♦ Analysis ♦ Female ♦ Humans ♦ Immunoassay ♦ Mice ♦ Pregnancy ♦ Toxicity Tests ♦ Journal Article
Abstract OBJECTIVE: The exact chemical composition of hydrosalpingeal fluid is unknown. The objective of this study was to characterize cytokines in hydrosalpingeal fluid (HSF) and examine their possible role in the embryo development. STUDY DESIGN: HSF was aspirated at laparoscopic salpingectomy in eight infertile women. Levels of IL-1beta, IL-13, IL-8, IL-6 and TNF-alpha in the HSF were determined by quantitative immunoassay kits. Two-cell mouse embryos were incubated with 0, 25, 50 and 75% concentrations of HSF. The blastocyst development rate (BDR) of mouse embryos was measured at each HSF concentration. RESULT(S): The embryotoxicity of HSF was concentration dependent. An increase in the HSF concentration resulted in significant decrease in % BDR (p < 0.01). IL-1beta was present in six of the eight HSF samples with a mean (+/-SD) concentration of 0.9 +/- 0.8 pg/mL. IL-13 was not detected in any of the HSF samples. IL-8, IL-6 and TNF-alpha were detected in all samples with a mean (+/-SD) concentration of 4741.2 +/- 6554.4 pg/mL, 204.8+/-132.8 pg/ml and 12 +/- 12.8 pg/mL respectively. IL-6 was positively correlated with BDR (r = 0.53; p < 0.04). CONCLUSION(S): We demonstrated for the first time the presence of IL-1beta, IL-8, IL-6 and TNF-alpha and the absence of IL-13 in human hydrosalpingeal fluid. IL-6 was positively related to the BDR.
Description Country affiliation: United States
Author Affiliation: Bedaiwy MA ( Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Department of Obstetrics & Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA. bedaiwymmm@yahoo.com)
ISSN 10580468
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2005-04-01
Publisher Place Netherlands
e-ISSN 15737330
Journal Journal of Assisted Reproduction and Genetics
Volume Number 22
Issue Number 4


Source: WHO-Global Index Medicus