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Author Yamada, Kyoko ♦ Fujita, Kazuyuki ♦ Quan, Jinhua ♦ Sekine, Masayuki ♦ Kashima, Katsunori ♦ Yahata, Tetsuro ♦ Tanaka, Kenichi
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Springer
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Diseases ♦ Gynecology, obstetrics, pediatrics & geriatrics
Subject Domain (in MeSH) Urogenital System ♦ Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Male Urogenital Diseases ♦ Diseases ♦ Cell Physiological Phenomena ♦ Genetic Phenomena ♦ Biological Sciences
Subject Keyword Discipline Reproductive Medicine ♦ Discipline Genectics ♦ Apoptosis ♦ Genetics ♦ Azoospermia ♦ Chromosomes, Human, Y ♦ Oligospermia ♦ Sequence Deletion ♦ Spermatozoa ♦ Cytology ♦ Humans ♦ Male ♦ Testis ♦ Pathology ♦ Journal Article
Abstract PURPOSE: AZFc deletions are associated with variable testicular histology ranging from the Sertoli cell only to spermatogenic arrest and hypospermatogenesis. Such variable phenotypes may be explained by progressive germ cell regression over time. Increased apoptosis is likely responsible for progressive regression of spermatogenic potential. This study evaluated germ cell apoptosis as a cause of the progressive decrease in the number of germ cells in patients with AZFc deletions. METHODS: This study evaluated germ cell apoptosis in patients with AZFc deletions. A total of 151 patients who were diagnosed with either severe oligozoospermia or non-obstructive azoospermia were screened for Y chromosome microdeletions. Germ cell apoptosis was examined using terminal deoxy-nucleotidyl transferase-mediated digoxigenin-dUTP nick-end labeling (TUNEL) on formalin-fixed 5-microm sections of testicular specimens. RESULTS: Seven out of 117 (6.0%) patients with azoospermia and 4 of 34 (11.8%) patients with severe oligozoospermia had Y chromosome microdeletions. The percentage of apoptotic germ cells in the testes of patients with AZFc deletions were significantly increased compared to those of patients without AZFc deletions. CONCLUSIONS: These results suggest that increased apoptosis of germ cells is responsible for the progressive decline of spermatogenic potential in patients with AZFc deletions.
Description Country affiliation: Japan
Author Affiliation: Yamada K ( Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-Dori, Niigata, 951-8520, Japan.)
ISSN 10580468
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-06-01
Publisher Place Netherlands
e-ISSN 15737330
Journal Journal of Assisted Reproduction and Genetics
Volume Number 27
Issue Number 6

Source: WHO-Global Index Medicus