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Author Mitsui, Akinori ♦ Yoshizawa, Midori ♦ Matsumoto, Hiromichi ♦ Fukui, Emiko
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Springer
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Social sciences ♦ Social problems & services; associations ♦ Social welfare problems & services ♦ Natural sciences & mathematics ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Gynecology, obstetrics, pediatrics & geriatrics ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Urogenital System ♦ Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Female Urogenital Diseases and Pregnancy Complications ♦ Diseases ♦ Therapeutics ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Cell Physiological Phenomena ♦ Physiological Phenomena ♦ Reproductive and Urinary Physiological Phenomena ♦ Biological Sciences ♦ Health Care Quality, Access, and Evaluation ♦ Health Care
Subject Keyword Discipline Reproductive Medicine ♦ Discipline Genectics ♦ Aging ♦ Genetics ♦ Chromosomes, Mammalian ♦ Embryonic Development ♦ Infertility, Female ♦ Therapy ♦ Nuclear Transfer Techniques ♦ Age Factors ♦ Animals ♦ Cytoplasm ♦ Physiology ♦ Female ♦ Fertilization In Vitro ♦ Methods ♦ Physiopathology ♦ Male ♦ Meiosis ♦ Mice ♦ Oocytes ♦ Pregnancy ♦ Pregnancy Rate ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract PURPOSE: The effects of reciprocal transplantation of meiosis-II chromosomes between senescent and young mouse oocytes were evaluated based on pre- and post-implantation development ability of resultant embryos. METHODS: Karyoplasts including meiosis-II chromosomes of oocytes from senescent Rockefeller mouse/Ms-Rb(6, 15) females (10 to 12 months, age-related infertile mice) were transferred into cytoplasts of oocytes from young F(1) females (3 to 5 months). Reconstructed oocytes were fertilized in vitro, and then the resultant embryos were cultured in vitro and transferred to recipient mice. RESULTS: The reconstructed oocytes that consisted of aged-karyoplasts and young-cytoplasts showed significantly improved embryonic development (from 23.2% to 30.0%) and development to term (from 6.3% to 27.1%, P < 0.05) as compared with the oocytes reconstructed from young-karyoplasts and aged-cytoplasts. CONCLUSIONS: The present study showed successful rejuvenation for age-related infertility using transplantation of meiosis-II chromosomes in animal experimental models.
Description Country affiliation: Japan
Author Affiliation: Mitsui A ( United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo, Japan. akinori.mitsui@kyowa-kirin.co.jp)
ISSN 10580468
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2009-01-01
Publisher Place Netherlands
e-ISSN 15737330
Journal Journal of Assisted Reproduction and Genetics
Volume Number 26
Issue Number 1


Source: WHO-Global Index Medicus