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Author Zhandi, Mahdi ♦ Towhidi, Armin ♦ Nasr-Esfahani, Mohammad Hossein ♦ Eftekhari-Yazdi, Poopak ♦ Zare-Shahneh, Ahmad
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Springer
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Gynecology, obstetrics, pediatrics & geriatrics ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Urogenital System ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Wounds and Injuries ♦ Diseases ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Cell Physiological Phenomena ♦ Genetic Phenomena ♦ Reproductive and Urinary Physiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Reproductive Medicine ♦ Discipline Genectics ♦ Cattle ♦ Physiology ♦ Heat Stress Disorders ♦ Veterinary ♦ Insulin-like Growth Factor I ♦ Pharmacology ♦ Oocytes ♦ Drug Effects ♦ Animals ♦ Apoptosis ♦ Dna Fragmentation ♦ Female ♦ Pathology ♦ In Situ Nick-end Labeling ♦ Male ♦ Growth & Development ♦ Pregnancy ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract PURPOSE: To investigate the effects of Insulin like growth factor-1 (IGF-1) on bovine oocyte developmental competence under heat stress. METHODS: In Experiment 1, bovine cumulus-oocyte complexes (COCs) were cultured at 38.5 or 41 degrees C for the first 12 h of maturation in the presence of either 100 ng/ml human recombinant (hr)-IGF-1 or acetic acid. In Experiment 2, COCs were cultured in 38.5 or 41 degrees C for the first 12 h of maturation in the presence either of 100 ng/ml hr-IGF-1 or acetic acid. After fertilization, putative zygotes were cultured for 8 days. RESULTS: In experiment 1, addition of rh-IGF-1 to maturation medium at 38.5 degrees C significantly increased the proportion of M II oocytes and decreased the percentage of TUNEL-positive oocytes compared to the other groups. However, addition of rh-IGF-1 to maturation medium under heat stress increased the percentage of TUNEL-positive oocytes. In experiment 2, addition of rh-IGF-1 under heat sress did not affect cleavage rate, whereas, blastocyst formation rate decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups. Similarly, The number of trophectoderm cells and total cell number were decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups and the percentage of TUNEL-positive nuclei were increased in heat-stressed and heat-stressed plus rh-IGF-1 groups compared to the other groups. CONCLUSION: The results of the present study demonstrate that IGF-1 decreases oocyte developmental competence and total cell number and increases TUNEL-positive nuclei at heat stress condition. These unexpected results of IGF-1 during maturation period under heat stress condition warrant further optimizations and investigations.
Description Country affiliation: Iran
Author Affiliation: Zhandi M ( Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, POBox#4111, Karaj, Iran.
ISSN 10580468
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2009-11-01
Publisher Place Netherlands
e-ISSN 15737330
Journal Journal of Assisted Reproduction and Genetics
Volume Number 26
Issue Number 11-12

Source: WHO-Global Index Medicus