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Author Reichman, David E. ♦ Politch, Joseph ♦ Ginsburg, Elizabeth S. ♦ Racowsky, Catherine
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Springer
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Gynecology, obstetrics, pediatrics & geriatrics ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Urogenital System ♦ Cells ♦ Embryonic Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Therapeutics ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physiological Phenomena ♦ Reproductive and Urinary Physiological Phenomena ♦ Biological Sciences ♦ Persons ♦ Persons
Subject Keyword Discipline Reproductive Medicine ♦ Discipline Genectics ♦ Oocytes ♦ Growth & Development ♦ Sperm Injections, Intracytoplasmic ♦ Methods ♦ Adult ♦ Cells, Cultured ♦ Embryo, Mammalian ♦ Metabolism ♦ Embryonic Development ♦ Female ♦ Humans ♦ Ovulation Induction ♦ Pregnancy ♦ Pregnancy Rate ♦ Journal Article
Abstract PURPOSE: To investigate 24 h in vitro maturation (IVM) of cumulus-stripped immature oocytes from stimulated cycles. METHODS: 263 oocytes identified as immature after cumulus stripping for ICSI were subjected to in vitro maturation (IVM). Fertilization rates and reproductive outcomes of matured oocytes were compared against 234 in vivo matured sibling oocytes (IVO-MII-Sib) from the same cycles (n = 41). Day 2 embryo development was compared against 116 embryos from ICSI cycles having no IVM (IVO-Ext controls). RESULTS: While fertilization rates were similar between IVM and IVO-MII-Sib oocytes (62.1% vs. 64.0%, p = 0.9909), day 2 embryo quality was reduced in the IVM group compared with IVO-Ext controls as evidenced by fewer embryos having 4 cells (28.3% vs. 54.3%, p = 0.0026), low fragmentation (30.0% vs. 65.2%, p < 0.0001) or perfectly symmetric blastomeres (28.6% vs. 46.9%, p = 0.0371). 0 of 17 IVM embryos with known fate implanted. CONCLUSION: Efficacy of 24 h IVM for cumulus-stripped GV and MI oocytes for either clinical use or study of normal meiotic maturation is questionable.
Description Country affiliation: United States
Author Affiliation: Reichman DE ( Department of Obstetrics, Gynecology and Reproductive Biology, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA.)
ISSN 10580468
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-07-01
Publisher Place Netherlands
e-ISSN 15737330
Journal Journal of Assisted Reproduction and Genetics
Volume Number 27
Issue Number 7


Source: WHO-Global Index Medicus