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Author Natarajan, Rajesh ♦ Shankar, Madhira Bhawani ♦ Munuswamy, Deecaraman
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Springer
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Gynecology, obstetrics, pediatrics & geriatrics ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Urogenital System ♦ Embryonic Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Heterocyclic Compounds ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Therapeutics ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Metabolism ♦ Physiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Reproductive Medicine ♦ Discipline Genectics ♦ Antioxidants ♦ Pharmacology ♦ Blastocyst ♦ Drug Effects ♦ Embryonic Development ♦ Oocytes ♦ Sheep ♦ Embryology ♦ Alpha-tocopherol ♦ Animals ♦ Cytology ♦ Culture Media ♦ Embryo Culture Techniques ♦ Female ♦ Fertilization In Vitro ♦ Oxidative Stress ♦ Journal Article
Abstract PURPOSE: To determine the effects of -tocopherol supplementation to oocyte maturation media and embryo culture media on the yield of ovine embryos. METHODS: -tocopherol, at concentrations of 0, 50, 100, 200, 400 and 500 µM was supplemented to ovine oocyte or embryo culture media and cultured at 5% or 20% O(2) levels. Percentages of cleavage, morula and blastocyst, total cell count and comet assay were taken as indicators of developmental competence of embryos. RESULTS: 200 µM -tocopherol in embryo culture medium at 20% O(2) level significantly increased the rates of cleavage (P < 0.05), morulae (P < 0.05) and blastocyst (P < 0.01) formation and blastocyst total cell number (P < 0.01) when compared with control. The rates of blastocyst formation were also significantly higher in 100 µM (P < 0.01) and 400 µM (P < 0.05) supplemented groups than control. CONCLUSION: -tocopherol supplementation may enhance the in vitro developmental competence of ovine embryos by protecting them from oxidative damage.
Description Country affiliation: India
Author Affiliation: Natarajan R ( Department of Industrial Biotechnology, Dr MGR Educational and Research Institute - Dr MGR University, Maduravoyal, Chennai 600095, Tamilnadu, India. r_jesh2002@yahoo.com)
ISSN 10580468
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-08-01
Publisher Place Netherlands
e-ISSN 15737330
Journal Journal of Assisted Reproduction and Genetics
Volume Number 27
Issue Number 8


Source: WHO-Global Index Medicus