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Author Borges, Edson Júnior ♦ Rossi, Lia Mara ♦ Farah, Leila ♦ Guilherme, Patricia ♦ Rocha, Claudia C. ♦ Ortiz, Valdemar ♦ Iaconelli, Assumpto Júnior
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Springer
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Gynecology, obstetrics, pediatrics & geriatrics
Subject Domain (in MeSH) Cells ♦ Embryonic Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Pathological Conditions, Signs and Symptoms ♦ Diseases ♦ Therapeutics ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Cell Physiological Phenomena ♦ Reproductive and Urinary Physiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Reproductive Medicine ♦ Discipline Genectics ♦ Cell Nucleolus ♦ Ultrastructure ♦ Chromosomes ♦ Sperm Injections, Intracytoplasmic ♦ Methods ♦ Cell Division ♦ Chromosome Aberrations ♦ Embryo Transfer ♦ Embryo, Mammalian ♦ Fertilization ♦ Humans ♦ Journal Article
Abstract PURPOSE: To find an effective method to select embryos from ICSI with better chromosomal status when preimplantation-genetic-diagnosis (PGD) is not applied. METHODS: Several morpholological evaluations were done in same embryos. Embryos from ICSI were classified on day 1 according to pronuclear-nucleoli arrangement. On day 3, classification was done according to number, fragmentation, size and shape of cells. In some patients, embryos exhibiting good quality on day 3 (at least six regular blastomeres with cell fragmentation lower than 20%) were also submitted to PGD, irrespective to pronuclear-nucleoli morphology. RESULTS: Forty-two per cent of normally fertilized embryos showed pronuclear-nucleoli-good-morphology; from those, 86% were also classified as good quality on day 3. Good-quality embryos submitted to PGD have shown lower chromosomal abnormality rates when also classified as pronuclear-nucleoli-good-morphology. CONCLUSIONS: Pronuclear-nucleoli morphology seems to be correlated with PGD results. This criterion may prove useful for pre-select embryos with normal chromosomal package when PGD is not applied.
Description Country affiliation: Brazil
Author Affiliation: Borges EJ ( Fertility-Centro de Fertilização Assistida, São Paulo, Brazil.)
ISSN 10580468
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2005-03-01
Publisher Place Netherlands
e-ISSN 15737330
Journal Journal of Assisted Reproduction and Genetics
Volume Number 22
Issue Number 3


Source: WHO-Global Index Medicus