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Author Rodríguez, Lleretny ♦ Navarrete, Felipe I. ♦ Tovar, Heribelt ♦ Cox, José F. ♦ Castro, Fidel Ovidio
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Springer
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Gynecology, obstetrics, pediatrics & geriatrics ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Urogenital System ♦ Cells ♦ Embryonic Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Therapeutics ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment
Subject Keyword Discipline Reproductive Medicine ♦ Discipline Genectics ♦ Cattle ♦ Embryology ♦ Embryo, Mammalian ♦ Cytology ♦ Oocytes ♦ Physiology ♦ Animals ♦ Blastocyst ♦ Cells, Cultured ♦ Cloning, Molecular ♦ Embryo Transfer ♦ Female ♦ In Vitro Techniques ♦ Micromanipulation ♦ Nuclear Transfer Techniques ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract PURPOSE: In order to simplify cloning, a new method that does not require micromanipulators was used. We aimed to evaluate the developmental potential of two bovine cell lines upon cloning. MATERIALS AND METHODS: In vitro matured bovine oocytes, were released from zona pellucida, enucleated, fused to foetal or adult somatic donor cells. The reconstructed embryos were reprogrammed, activated and cultured until blastocyst stage. No micromanipulators were used. Blastocyst rate and quality was scored. Some expanded (d7) blastocysts were transferred to recipient cattle and collected back at d17 to assess elongation. RESULTS: High developmental potential in vitro of cloned embryos to expanded (d7) blastocysts was achieved (52.6%). In one cell line, 65.7% of blastocysts was scored. Most blastocysts (87.4%) were graded as excellent. In vivo development to elongation (day-17) in temporary recipient cows also showed a high developmental potential (11/18 transferred blastocysts elongated). CONCLUSIONS: Hand-made cloning is an efficient alternative for cloning in cattle.
Description Country affiliation: Chile
Author Affiliation: Rodríguez L ( Animal Science, University of Concepcion, Avenida Vicente Méndez 595, Chillán, 537, Chile.)
ISSN 10580468
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2008-01-01
Publisher Place Netherlands
e-ISSN 15737330
Journal Journal of Assisted Reproduction and Genetics
Volume Number 25
Issue Number 1

Source: WHO-Global Index Medicus