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Author Chen, Shanwen ♦ Zhu, Jing ♦ Zuo, Shuai ♦ Ma, Ju ♦ Zhang, Junling ♦ Chen, Guowei ♦ Wang, Xin ♦ Pan, Yisheng ♦ Liu, Yucun ♦ Wang, Pengyuan
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ACTIN ♦ CELL PROLIFERATION ♦ INHIBITION ♦ LARGE INTESTINE ♦ METASTASES ♦ NEOPLASMS ♦ SECRETION ♦ TRANSCRIPTION ♦ TRANSCRIPTION FACTORS
Abstract 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) has been reported to inhibit proliferation and migration of multiple types of cancer cells. However, the mechanism underlying its anti-metastasis effect is not fully illustrated. In this study, the effect of 1,25(OH)2D3 on TGF-β1/β2-induced epithelial–mesenchymal transition (EMT) is tested in colon cancer cells. The results suggest that 1,25(OH)2D3 inhibited TGF-β1/β2-induced increased invasion and migration of in SW-480 and HT-29 cells. 1,25(OH)2D3 also inhibited the cadherin switch in SW-480 and HT-29 cells. TGF-β1/β2-induced increased expression of EMT-related transcription factors was also inhibited by 1,25(OH)2D3. 1,25(OH)2D3 also inhibited the secretion of MMP-2 and MMP-9 and increased expression of F-actin induced by TGF-β1/β2 in SW-480 cells. Taken together, this study suggests that the suppression of EMT might be one of the mechanisms underlying the anti-metastasis effect of 1,25(OH)2D3 in colon cancer cells. - Highlights: • TGF-β1/β2-induced model of EMT was used in this study to test the effect of 1,25(OH)2D3 on EMT in colon cancer cells. • 1,25(OH)2D3 inhibited TGF-β1/β2-induced increased migration and invasion. • 1,25(OH)2D3 inhibited TGF-β1/β2-induced increased level of EMT-related transcription factors. • 1,25(OH)2D3 inhibited TGF-β1/β2-induced increased expression of F-actin in SW-480 cells.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-12-04
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 468
Issue Number 1-2


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