Thumbnail
Access Restriction
Open

Author Aherne, G. W. ♦ Marks, V.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BASIC BIOLOGICAL SCIENCES ♦ NUCLEOTIDES ♦ RADIOIMMUNOASSAY ♦ ANIMAL CELLS ♦ ANTIBODIES ♦ CHROMATOGRAPHY ♦ FIBROBLASTS ♦ LYMPHOMAS ♦ MICE ♦ OXIDATION ♦ PERIODATES ♦ QUANTITATIVE CHEMICAL ANALYSIS ♦ SENSITIVITY ♦ SODIUM COMPOUNDS ♦ ALKALI METAL COMPOUNDS ♦ ANIMALS ♦ CHEMICAL ANALYSIS ♦ CHEMICAL REACTIONS ♦ CONNECTIVE TISSUE CELLS ♦ DISEASES ♦ HALOGEN COMPOUNDS ♦ IMMUNOASSAY ♦ IMMUNOLOGY ♦ IODINE COMPOUNDS ♦ ISOTOPE APPLICATIONS ♦ MAMMALS ♦ NEOPLASMS ♦ ORGANIC COMPOUNDS ♦ OXYGEN COMPOUNDS ♦ RADIOASSAY ♦ RADIOIMMUNOLOGY ♦ RODENTS ♦ SEPARATION PROCESSES ♦ SOMATIC CELLS ♦ TRACER TECHNIQUES ♦ VERTEBRATES ♦ Biochemistry- Tracer Techniques
Abstract A radioimmunoassay (RIA) capable of quantitating dCTP in femtomolar amounts in cell extracts has been developed, and applied to human fibroblast cells lines and L5178Y mouse lymphoma lines. Cross reactivity of the antibody with CTP, though low (2.7%) has necessitated pre-RIA removal of CTP by either boronate affinity gel chromatography or sodium periodate oxidation. Fractions from the boronate gel column or aliquots of NaIO/sub 4/-treated cell extract are quantitated directly by the RIA. Recovery of extracted dCTP standard taken through the entire procedure is quantitative and results are reproductive. Due to the high sensitivity of the quantitation step, dCTP can be accurately measured in relatively small numbers of cells-about 10/sup 4/ cells.
Educational Use Research
Learning Resource Type Article
Publisher Date 1986-04-01
Publisher Place United States
Journal Anal. Biochem.
Volume Number 154
Issue Number 1
Organization Univ. of Surrey, England


Open content in new tab

   Open content in new tab