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Author Musselman, Inga H. ♦ Pooja, Bajaj ♦ Yehia, Hadi N. ♦ Daigrepont, Meredith C. ♦ Erin, Walker ♦ Dieckmann, Gregg R. ♦ Carole, Mikoryak ♦ Paul, Pantano ♦ Draper, Rockford K.
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher BioMed Central
File Format HTM / HTML
Date Created 2007-12-23
Copyright Year ©2007
Language English
Subject Domain (in LCC) TP248.13-248.65 ♦ R855-855.5
Subject Keyword Biotechnology ♦ Medicine ♦ Chemical technology ♦ Technology ♦ Medical technology
Abstract Abstract This work concerns exposing cultured human epithelial-like HeLa cells to single-walled carbon nanotubes (SWNTs) dispersed in cell culture media supplemented with serum. First, the as-received CoMoCAT SWNT-containing powder was characterized using scanning electron microscopy and thermal gravimetric analyses. Characterizations of the purified dispersions, termed DM-SWNTs, involved atomic force microscopy, inductively coupled plasma – mass spectrometry, and absorption and Raman spectroscopies. Confocal microRaman spectroscopy was used to demonstrate that DM-SWNTs were taken up by HeLa cells in a time- and temperature-dependent fashion. Transmission electron microscopy revealed SWNT-like material in intracellular vacuoles. The morphologies and growth rates of HeLa cells exposed to DM-SWNTs were statistically similar to control cells over the course of 4 d. Finally, flow cytometry was used to show that the fluorescence from MitoSOX™ Red, a selective indicator of superoxide in mitochondria, was statistically similar in both control cells and cells incubated in DM-SWNTs. The combined results indicate that under our sample preparation protocols and assay conditions, CoMoCAT DM-SWNT dispersions are not inherently cytotoxic to HeLa cells. We conclude with recommendations for improving the accuracy and comparability of carbon nanotube (CNT) cytotoxicity reports.
ISSN 14773155
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2007-10-01
e-ISSN 14773155
Journal Journal of Nanobiotechnology
Volume Number 5
Issue Number 1
Starting Page 8

Source: Directory of Open Access Journals (DOAJ)