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Author Chapman, Michael S.
Sponsorship USDOE
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY ♦ DATA PROCESSING ♦ DIFFRACTION ♦ ICE ♦ CORRECTIONS
Abstract Macromolecular structures are routinely determined at cryotemperatures using samples flash-cooled in the presence of cryoprotectants. However, sometimes the best diffraction is obtained under conditions where ice formation is not completely ablated, with the result that characteristic ice rings are superimposed on the macromolecular diffraction. In data processing, the reflections that are most affected by the ice rings are usually excluded. Here, an alternative approach of subtracting the ice diffraction is tested. High completeness can be retained with little adverse effect upon the quality of the integrated data. This offers an alternate strategy when high levels of cryoprotectant lead to loss of crystal quality.
Educational Use Research
Learning Resource Type Article
Publisher Date 2010-11-03
Publisher Place United States
Journal Acta Crystallogr. D
Volume Number 66
Issue Number 6
Organization Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)


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