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Author Johnson, A. E. ♦ Ball, G. F. ♦ Coirini, H. ♦ Harbaugh, C. R. ♦ McEwen, B. S. ♦ Insel, T. R.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BASIC BIOLOGICAL SCIENCES ♦ OXYTOCIN ♦ RECEPTORS ♦ BIOCHEMICAL REACTION KINETICS ♦ ANGIOTENSIN ♦ AUTORADIOGRAPHY ♦ ESTRADIOL ♦ IODINE 125 ♦ OVARIES ♦ RATS ♦ UTERUS ♦ WEIGHT ♦ ANIMALS ♦ BETA DECAY RADIOISOTOPES ♦ BODY ♦ CARDIOVASCULAR AGENTS ♦ DAYS LIVING RADIOISOTOPES ♦ DRUGS ♦ ELECTRON CAPTURE RADIOISOTOPES ♦ ESTRANES ♦ ESTROGENS ♦ FEMALE GENITALS ♦ GLOBULINS ♦ GONADS ♦ HORMONES ♦ HYDROXY COMPOUNDS ♦ INTERMEDIATE MASS NUCLEI ♦ IODINE ISOTOPES ♦ ISOTOPES ♦ KINETICS ♦ MAMMALS ♦ MEMBRANE PROTEINS ♦ NUCLEI ♦ ODD-EVEN NUCLEI ♦ ORGANIC COMPOUNDS ♦ ORGANS ♦ PEPTIDE HORMONES ♦ PITUITARY HORMONES ♦ PROTEINS ♦ RADIOISOTOPES ♦ REACTION KINETICS ♦ RODENTS ♦ STEROID HORMONES ♦ STEROIDS ♦ VASOCONSTRICTORS ♦ VERTEBRATES ♦ Biochemistry- Tracer Techniques
Abstract Oxytocin (OT) transmission is involved in the steroid-dependent display of sexual receptivity in rats. One of the biochemical processes stimulated by the ovarian steroid 17 beta-estradiol (E2) that is relevant to reproduction is the induction of OT receptor binding in the ventromedial hypothalamic nucleus (VMN). The purpose of these experiments was to determine if E2-induced changes in OT receptor binding in the VMN occur within a time frame relevant to cyclic changes in ovarian steroid secretion. OT receptor binding was measured in the VMN of ovariectomized rats implanted for 0-96 h with E2-containing Silastic capsules. The rate of decay of OT receptor binding was measured in another group of animals 6-48 h after capsule removal. Receptors were labeled with the specific OT receptor antagonist ({sup 125}I)d(CH2)5(Tyr(Me)2,Thr4,Tyr-NH2(9))OVT, and binding was measured with quantitative autoradiographic methods. In addition, plasma E2 levels and uterine weights were assessed in animals from each treatment condition. Significant increases in E2-dependent OT receptor binding and uterine weight occurred within 24 h of steroid treatment. After E2 withdrawal, OT receptor binding and uterine weight decreased significantly within 24 h. These results are consistent with the hypothesis that steroid modulation of OT receptor binding is necessary for the induction of sexual receptivity.
ISSN 00137227
Educational Use Research
Learning Resource Type Article
Publisher Date 1989-09-01
Publisher Place United States
Journal Endocrinology
Volume Number 125
Issue Number 3


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