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Author Kim, Sanghoon ♦ Yu, Hyuk
Sponsorship USDOE
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword CHEMISTRY ♦ BIOLOGY AND MEDICINE, BASIC STUDIES ♦ POLYMERS ♦ DIFFUSION ♦ PROTEINS ♦ PHOSPHOLIPIDS ♦ AIR-WATER INTERACTIONS
Abstract We report a lateral diffusion study on the air/water interface of a surface-active protein, bacterial lipase from Pseudomonas fluorescens, and vinyl polymer, poly(tert-butyl methacrylate), with the technique of fluorescence recovery after photobleaching. For the validation of implementating the technique and the calibration of our instrument, we relied on a phospholipid system that Peters and Beck have used earlier and found that our results were in accord with theirs within 20% in absolute magnitude. For both the phospholipid and lipase, we analyzed the lateral diffusion data in terms of the free area model of Sackmann and Traeuble. We conclude that the results of lipase could be interpreted by invoking a conformational change induced by lateral compression in the monolayer state and those of the polymer, by postulating the quenching of the diffusion process when its concentration crosses over from the dilute regime to the semidilute regime. 37 refs., 11 figs., 2 tabs.
ISSN 00223654
Educational Use Research
Learning Resource Type Article
Publisher Date 1992-05-14
Publisher Place United States
Journal Journal of Physical Chemistry
Volume Number 96
Issue Number 10


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