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Author Mittal, Deepika Rani
Researcher Mittal, Deepika Rani
Source NIT Rourkela-Thesis
Content type Text
Educational Degree Bachelor of Technology (B.Tech.)
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword Biomedical Engineering
Abstract In the present investigation, we demonstrated the nature of interaction between silver nanoparticles and E.Coli bacterial. Stable silver nanoparticles (NP) were prepared by green synthesis (Catharanthus plant extract) method and characterized by UV-Vis spectrophotometry, SEM and DLS (Dynamic Light Scattering) particle size analysis.The antimicrobial activity of silver nanoparticles was monitored against Escherichia coli (Gram negative bacteria). Nutrient broth solutions were used to culture the Escherichia coli and silver nanoparticles of different concentrations were added to the bacterial culture solution to monitor toxicity of NP and simultaneously to investigate cell-NP interaction. It was observed that Escherichia coli growth was inhibited at a NP concentration and 80μg/ml concentration NP was found to kill E.coli completely.
Education Level UG and PG
Learning Resource Type Thesis
Publisher Date 2011-01-01