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Author Morimoto, K. ♦ Fukuda, J. ♦ Suzuki, H.
Source IEEE Xplore Digital Library
Content type Text
Publisher Institute of Electrical and Electronics Engineers, Inc. (IEEE)
File Format PDF
Copyright Year ©2007
Language English
Subject Domain (in DDC) Technology ♦ Engineering & allied operations ♦ Applied physics
Subject Keyword Biochemistry ♦ Electrodes ♦ Substrates ♦ Glass ♦ Actuators ♦ Electrochemical processes ♦ System-on-a-chip ♦ Negative feedback ♦ Current measurement ♦ Charge measurement
Abstract Aiming for the application to an ingestible micro system, a microanalysis system for the determination of the activity of digestive enzymes was realized. The analysis was conducted by using an on-chip micro-pH-stat which worked on the basis of an irregular use of the electrochemical three-electrode system. The operation using a potentiostat caused a negative feedback, maintaining the solution pH at a constant value. The system used a freeze-dried enzyme substrate. The very porous matrix facilitated rapid dissolution and mixing with the enzyme solution. The determination of the activity of pepsin and trypsin was conducted at the optimum pHs for the respective enzymes by measuring the generated charge accompanying the hydrolysis of hemoglobin. In both cases, a distinct increase in the generated charge was observed as the enzymatic reaction proceeded. Linear relationships were observed between the initial rate of charge increase and the activity of the enzymes.
Description Author affiliation: Univ. of Tsukuba, Ibaraki (Morimoto, K.; Fukuda, J.; Suzuki, H.)
ISBN 9781424412617
ISSN 19300395
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research ♦ Reading
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2007-10-28
Publisher Place USA
Rights Holder Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Size (in Bytes) 447.64 kB
Page Count 4
Starting Page 458
Ending Page 461


Source: IEEE Xplore Digital Library