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Author Nock, V. ♦ Blaikie, R.J.
Source IEEE Xplore Digital Library
Content type Text
Publisher Institute of Electrical and Electronics Engineers, Inc. (IEEE)
File Format PDF
Copyright Year ©2009
Language English
Subject Domain (in DDC) Technology ♦ Engineering & allied operations ♦ Applied physics
Subject Keyword Visualization ♦ Fluid flow measurement ♦ Hydrodynamics ♦ Microfluidics ♦ Oxygen ♦ Character generation ♦ Solid state circuits ♦ Sensor phenomena and characterization ♦ Microchannel ♦ Biological cells
Abstract Oxygen is an important parameter with significant effect on cellular development and function. Based on microfluidics, laminar flow and hydrodynamic focusing provide ideal tools for the generation of controlled micro-environments and their application as stimuli to cells. In this paper we demonstrate, for the first time inside a microfluidic device, the generation and characterization of hydrodynamically focused streams with defined dissolved oxygen level. A solid-state oxygen sensor layer was integrated into a PDMS-based microchannel and calibrated. Several combinations of sample and buffer streams with concentrations ranging from 0 to 34 mg/L O were generated and measured down to a sample stream width of 20 µm. The device has the potential to provide novel insights into cell biology and improve the relevance of in-vitro cell assays.
Description Author affiliation: MacDiarmid Institute for Advanced Materials and Nanotechnology, Department for Electrical and Computer Engineering University of Canterbury, Christchurch, New Zealand (Nock, V.; Blaikie, R.J.)
ISBN 9781424445486
ISSN 19300395
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research ♦ Reading
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2009-10-25
Publisher Place New Zealand
Rights Holder Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Size (in Bytes) 710.54 kB
Page Count 4
Starting Page 1248
Ending Page 1251


Source: IEEE Xplore Digital Library